Ovarian tissue vitrification is more efficient than slow freezing in protecting oocyte and granulosa cell DNA integrity
نویسندگان
چکیده
منابع مشابه
Human ovarian tissue: vitrification versus conventional freezing.
Sir, In the challenging paper by Wang et al. (2008), the authors have reported about an effective method of vitrification of human ovarian tissue with direct contact of cells in liquid nitrogen, named needle immersed vitrification. Childbirth after cryopreservation of ovarian tissue is now a reality (Donnez et al., 2004; Demeestere et al., 2007; Meirow et al., 2007; Andersen et al., 2008) and t...
متن کاملI-10: Vitrification of Baboon Ovarian Tissue:Will Vitrification Replace Conventional Freezing for Ovarian Tissue Cryopreservation?
Background: The aim of this study was to evaluate the efficacy of a vitrification protocol developed by our group to cryopreserve ovarian tissue. For this, we used ovaries from non-human primates in order to have an animal model close to the clinical setting. Materials and Methods: Ovarian biopsies from five adult baboons were vitrified, warmed and autografted. After five months, follicle survi...
متن کاملVitrification of human ovarian tissue: a practical and relevant alternative to slow freezing
BACKGROUND Cryopreservation of ovarian tissue can be used to preserve the fertility of patients who are about to receive treatment(s) that could compromise their future ovarian function. Here we evaluate the effectiveness of a vitrification protocol by carrying out a systematic comparison with a conventional slow-freezing method on human ovarian tissue. METHODS Human ovarian samples (mean age...
متن کاملVitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue.
BACKGROUND Controlled-rate freezing of ovarian cortical tissue for preservation of fertility among young women facing chemo- or radio-therapy is a widely accepted procedure. To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus slow programmed freezing. METHODS Ovarian tissue from 20 women, donated...
متن کاملProspective randomized comparison of human oocyte cryopreservation with slow-rate freezing or vitrification.
OBJECTIVE To compare cryopreservation of mature human oocytes with slow-rate freezing and vitrification and determine which is most efficient at establishing a pregnancy. DESIGN Prospective randomized. SETTING Academically affiliated, private fertility center. PATIENT(S) Consenting patients with concerns about embryo cryopreservation and more than nine mature oocytes at retrieval were ran...
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ژورنال
عنوان ژورنال: Systems Biology in Reproductive Medicine
سال: 2014
ISSN: 1939-6368,1939-6376
DOI: 10.3109/19396368.2014.923542